本文構(gòu)建具有DNA 聚合酶鏈?zhǔn)椒磻?yīng)（PCR）和毛細(xì)管電泳(CE)分離檢測功能的微流體芯片uC/OS-II嵌入式實(shí)時(shí)控制系統(tǒng)。本系統(tǒng)采用32 位嵌入式微控制器ARM 實(shí)現(xiàn)PCR 擴(kuò)增所需的閉環(huán)溫度控制功能，毛細(xì)管電泳分離功能所需的高壓電場自動(dòng)調(diào)度功能。uC/OS-II 嵌入式實(shí)時(shí)操作系統(tǒng)加強(qiáng)了系統(tǒng)的實(shí)時(shí)性。模糊免疫PID 控制算法實(shí)現(xiàn)對(duì)溫度的精確控制，其控制性能遠(yuǎn)優(yōu)于常規(guī)PID 控制器。試驗(yàn)結(jié)果表明本系統(tǒng)不但完全滿足設(shè)計(jì)要求，而且與同功能的儀器平臺(tái)相比，實(shí)時(shí)性強(qiáng)，不再需要昂貴的個(gè)人電腦進(jìn)行控制運(yùn)算和常用的DNA 檢測設(shè)備進(jìn)行檢測，實(shí)現(xiàn)了PCR-CE 微流體芯片系統(tǒng)的低成本和小型化。
關(guān)鍵詞：微流體芯片；ARM； 模糊免疫PID 算法
Real Time Embedded System in Micro-fluidic Chips Based on the uC/OS-II kernel WANG-haifeng 1 ，ZHANG-haili 2 ， ZHANG -yulin1 (1.School of Control Science and Engineering ，Shandong University，250061，Jinan, China; 2.Automation Lab , Shandong Institute of Light Industry, 250100，Jinan, China) Abstract This paper has developed integrated polymerase chain reaction - capillary electrophoresis (PCR-CE) micro-fluidic chips real-time embedded system based on uC/OS-II, can perform a series of analytical steps ranging from sample pre-concentration, PCR and CE separation. The miniaturized device platform has been designed based on the embedded, 32 bites RISC microcontroller ARM. Because the ability to precisely control temperature is a critical component of PCR micro-fluidic devices, the fuzzy immune PID control arithmetic is used to ensure the temperature control precise and robust. The platform includes matrix
keyboard, small LCD displayer, serial communication unit, electroosmosis flow unit and
PCR unit,. The miniaturized device platform can not only real-time fulfill all the functions
in the PCR-CE micro- fluidic chips without the help of expensive personal computers and
other measure devices but also realize the micro-fluidic chips system low cost and
miniaturization.
Keywords: Micro-fluidic chip ; uC/OS-II; ARM ; Fuzzy immune PID